The 6th European Medical and Biological Engineering Conference (MBEC 2014) was sponsored by the International Federation for Medical and Biological Engineering (IFMBE) in Dubrovnik, Croatia, September 7-11, 2014. This conference continues the series of well-known European IFMBE Conferences held in Budapest in 2011, Antwerp in 2008, Prague in 2005, and two times in Vienna in 2002 and 1999. The general theme of MBEC 2014 was “Towards new horizons in biomedical engineering”. Professor Bruce Wheeler was invited to give a keynote talk for the conference on “Cultured Neural Networks: From Patterns to Circuits with Information Transfer”.