BME Core Facilities

Kelley Hines
Manager of Core and Laboratory Infrastructure
(352) 273-7231
KHines@bme.ufl.edu

 

 

The equipment described below is shared instrumentation available in the Biomedical Sciences Building:

Sorvall WX Ultra 90 Floor Ultra Centrifuge
Located in BMS, J361
This high performance ultra centrifuge will allow nanoparticle separation at up to 90,000 rpm.

Sorvall RC 6 Plus Superspeed Centrifuge
Located in BMS, J243
This high speed, high capacity centrifuge offers 4 liters of capacity and processing speeds of up to 22,000 rpm enabling fast processing of samples.

Zeiss LSM710 Confocal Microscope
Located in BMS, J238A
This high precision confocal microscope comes with 5 lasers (405, 488, 514, 546, 688 nm) and offers great sensitivity and less noise, due to longer sampling time per pixel and excellent contrast characteristics.

Zeiss High Resolution Inverted Fluorescence Microscope
Located in BMS, J238A
This device offers brightfield, darkfield, phase contrast, differential interference contrast and fluorescence (FITC, Cy3, Cy5 and DAPI) and provides sharp image contrasts for clear imaging.

Molecular Devices M5E Plate Reader
Located in BMS, J223
This is a temperature controlled (4°C above ambient to 50°C) plate reader that can measure fluorescence, luminescence, absorbance, time-resolved fluorescence (TRF) and fluorescence polarization (TRP). It has dual monochromators that allows users to determine optimal excitation and emission settings for both fluorescence and absorbance. This instrument can handle 6-, 96-, and 384- well plates and allows for end point, kinetic, spectral scanning, and well-area scanning modes.

Perkin Elmer IVIS Spectrum CT In Vivo Imaging System
Located in BMS, J320
This is an in vivo imaging system to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in live animals. It also offers single-view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context. It offers High resolution (to 20 microns) with 3.9 cm field of view, with twenty eight high efficiency filters spanning 430 – 850 nm. This instrument can do 3D diffuse tomographic reconstruction for both fluorescence and bioluminescence.

Zeiss LSM 880 Confocal Microscope with Airy Scan
Located in BMS, J320
This device provides high resolution, sensitivity and speed that increases productivity when performing quantitative imaging. Its unique Airyscan detector enables superresolution imaging (120 nm resolution in XY, 400 nm in Z). It includes laser lines 405, 458, 488, 514, 561, 633 nm.

LI-COR Odyssey CLx Imaging Station
Located in BMS, J228
This is an infrared imager features two spectrally separate solid state lasers (685 nm and 785 nm) to detect infrared dye-labeled conjugates and near-infrared stains. This device generates imaging results in the much cleaner background, higher signal-to-noise ratios and better sensitivity, as compared to standard imaging system.

BD FACS Celesta Flow Cytometer
Located in BMS, J228
This is a multicolor flow cytometer that comes with 3 lasers (blue, red and violet) and can be employed in cell counting and biomarker detection.

Leica CryoStat CM1950
Located in BMS, J217
This is a highly adaptable device used to do frozen section histology at a temperature as low as -20 degrees C.

Bruker Microindentor
Located in BMS, J219
This is tool for determining the mechanical properties, such as the hardness, of a sample.

General Data ETC 2120 Histology Embedding Station
Located in BMS, J220
This equipment embed tissue samples and create paraffin embedded tissue blocks.

Leica Rotary Microtome RM2125 RTS
Located in BMS, J217
This manually operated device is used for creating thin sections of specimens of varying hardness. Is designed for sectioning soft paraffin specimens.

Thermo Fisher CryoStat HM550
Located in BMS, J217
This is a highly adaptable device used to do frozen section histology at a temperature as low as -20 degrees C.

Thermo HM 650v Vibration Microtome
Located in BMS, J217
This device offers vibrating blad that is designed for sectioning fresh tissue, such as spinal cord and brain, as well as for sectioning fixed tissue.