The objective of this research was to prepare a chitosan nano test tube drug delivery system.  Defect-free, chitosan nano test tubes of uniform size were synthesized within the pores of a nanoporous alumina template.  While the nano test tubes remained embedded in the template, their inner cavities were filled with a payload.  The payload was then trapped inside the nano test tubes by sealing the open ends of the tubes with latex nanoparticle caps.  Once removed from the template, the exterior surfaces of the nano test tubes were modified with antibodies in order to develop a targetable system.  Since chitosan is a biodegradable polymer, once the nano test tubes reach their target site and are taken into a cell, the tubes can be degraded and thus cause the release of the inner payload.  This chitosan nano test tube delivery system shows great potential for applications in targeted drug delivery.